Bactopia Tool - gtdb

The gtdb tool uses GTDB-Tk's classify workflow to assign taxonomic classifications to your set of samples. This is done through the use of the Genome Taxonomy Database. If you are unsure of your sequences, gtdb is useful tool to help determine the taxonomy of your samples.

Example Usage

bactopia --wf gtdb \
  --bactopia /path/to/your/bactopia/results  

Output Overview

Below is the default output structure for the gtdb tool. Where possible the file descriptions below were modified from a tools description.

<BACTOPIA_DIR>
├── <SAMPLE_NAME>
│   └── tools
│       └── gtdb
│           ├── align
│           │   ├── <SAMPLE_NAME>.{ar122|bac120}.filtered.tsv
│           │   ├── <SAMPLE_NAME>.{ar122|bac120}.msa.fasta.gz
│           │   └── <SAMPLE_NAME>.{ar122|bac120}.user_msa.fasta.gz
│           ├── classify
│           │   ├── <SAMPLE_NAME>.{ar122|bac120}.classify.tree.1.tree
│           │   ├── <SAMPLE_NAME>.{ar122|bac120}.classify.tree.6.tree
│           │   ├── <SAMPLE_NAME>.{ar122|bac120}.summary.tsv
│           │   ├── <SAMPLE_NAME>.{ar122|bac120}.tree.mapping.tsv
│           │   └── <SAMPLE_NAME>.backbone.{ar122|bac120}.classify.tree
│           ├── gtdbtk.json
│           ├── identify
│           │   ├── <SAMPLE_NAME>.ar53.markers_summary.tsv
│           │   ├── <SAMPLE_NAME>.bac120.markers_summary.tsv
│           │   ├── <SAMPLE_NAME>.failed_genomes.tsv
│           │   └── <SAMPLE_NAME>.translation_table_summary.tsv
│           ├── logs
│           │   ├── nf-gtdb.{begin,err,log,out,run,sh,trace}
│           │   ├── results
│           │   │   ├── gtdbtk.log
│           │   │   └── gtdbtk.warnings.log
│           │   └── versions.yml
│           └── <SAMPLE_NAME>.{ar122|bac120}.summary.tsv
└── bactopia-runs
    └── gtdb-<TIMESTAMP>
        ├── merged-results
        │   ├── gtdb.tsv
        │   └── logs
        │       └── gtdb-concat
        │           ├── nf-merged-results.{begin,err,log,out,run,sh,trace}
        │           └── versions.yml
        └── nf-reports
            ├── gtdb-dag.dot
            ├── gtdb-report.html
            ├── gtdb-timeline.html
            └── gtdb-trace.txt

Results

GTDB-Tk

Below is a description of the per-sample results from GTDB-Tk. For full details about each of the GTDB output files, see GTDB-Tk Classify Workflow page.

Filename	Description
<SAMPLE_NAME>.{ar122	bac120}.classify.tree
<SAMPLE_NAME>.{ar122	bac120}.filtered.tsv
<SAMPLE_NAME>.{ar122	bac120}.markers_summary.tsv
<SAMPLE_NAME>.{ar122	bac120}.msa.fasta
<SAMPLE_NAME>.{ar122	bac120}.summary.tsv
<SAMPLE_NAME>.{ar122	bac120}.user_msa.fasta
<SAMPLE_NAME>.translation_table_summary.tsv	Summary of the translation table used for each genome
<SAMPLE_NAME>.ar122.markers_summary.tsv	Summary of unique, duplicated, and missing markers within the 122 archaeal marker set for each submitted genome
<SAMPLE_NAME>.bac120.markers_summary.tsv	Summary of unique, duplicated, and missing markers within the 120 bacterial marker set for each submitted genome

Audit Trail

Below are files that can assist you in understanding which parameters and program versions were used.

Logs

Each process that is executed will have a folder named logs. In this folder are helpful files for you to review if the need ever arises.

Extension	Description
.begin	An empty file used to designate the process started
.err	Contains STDERR outputs from the process
.log	Contains both STDERR and STDOUT outputs from the process
.out	Contains STDOUT outputs from the process
.run	The script Nextflow uses to stage/unstage files and queue processes based on given profile
.sh	The script executed by bash for the process
.trace	The Nextflow Trace report for the process
versions.yml	A YAML formatted file with program versions

Nextflow Reports

These Nextflow reports provide great a great summary of your run. These can be used to optimize resource usage and estimate expected costs if using cloud platforms.

Filename	Description
gtdb-dag.dot	The Nextflow DAG visualisation
gtdb-report.html	The Nextflow Execution Report
gtdb-timeline.html	The Nextflow Timeline Report
gtdb-trace.txt	The Nextflow Trace report

Program Versions

At the end of each run, each of the versions.yml files are merged into the files below.

Filename	Description
software_versions.yml	A complete list of programs and versions used by each process
software_versions_mqc.yml	A complete list of programs and versions formatted for MultiQC

Parameters

Required Parameters

Define where the pipeline should find input data and save output data.

Parameter	Description
--bactopia	The path to bactopia results to use as inputs Type: string

Filtering Parameters

Use these parameters to specify which samples to include or exclude.

Parameter	Description
--include	A text file containing sample names (one per line) to include from the analysis Type: string
--exclude	A text file containing sample names (one per line) to exclude from the analysis Type: string

GTDB Setup Parameters

Parameter	Description
--gtdb	Tarball or path of a GTDB database. If a database is not found, you must use '--download_gtdb' Type: string
--download_gtdb	Download the latest GTDB database, even it exists Type: boolean
--gtdb_save_as_tarball	Download the latest GTDB database, and save it in a single tarball Type: boolean
--skip_check	Do not check the installation of GTDB database Type: boolean

GTDB Classify Parameters

Parameter	Description
--min_af	Minimum alignment fraction to consider closest genome Type: number, Default: 0.65
--min_perc_aa	Filter genomes with an insufficient percentage of AA in the MSA Type: integer, Default: 10
--gtdb_tmp	Specify alternative directory for temporary files Type: boolean, Default: /tmp
--gtdb_use_scratch	Reduce pplacer memory usage by writing to --gtdb_tmp location (slower) Type: boolean
--gtdb_debug	Create intermediate files for debugging purposes Type: boolean
--force_gtdb	Continue processing if an error occurs on a single genome Type: boolean

Optional Parameters

These optional parameters can be useful in certain settings.

Parameter	Description
--outdir	Base directory to write results to Type: string, Default: bactopia
--skip_compression	Ouput files will not be compressed Type: boolean
--datasets	The path to cache datasets to Type: string
--keep_all_files	Keeps all analysis files created Type: boolean

Max Job Request Parameters

Set the top limit for requested resources for any single job.

Parameter	Description
--max_retry	Maximum times to retry a process before allowing it to fail. Type: integer, Default: 3
--max_cpus	Maximum number of CPUs that can be requested for any single job. Type: integer, Default: 4
--max_memory	Maximum amount of memory (in GB) that can be requested for any single job. Type: integer, Default: 32
--max_time	Maximum amount of time (in minutes) that can be requested for any single job. Type: integer, Default: 120
--max_downloads	Maximum number of samples to download at a time Type: integer, Default: 3

Nextflow Configuration Parameters

Parameters to fine-tune your Nextflow setup.

Parameter	Description
--nfconfig	A Nextflow compatible config file for custom profiles, loaded last and will overwrite existing variables if set. Type: string
--publish_dir_mode	Method used to save pipeline results to output directory. Type: string, Default: copy
--infodir	Directory to keep pipeline Nextflow logs and reports. Type: string, Default: ${params.outdir}/pipeline_info
--force	Nextflow will overwrite existing output files. Type: boolean
--cleanup_workdir	After Bactopia is successfully executed, the work directory will be deleted. Type: boolean

Institutional config options

Parameters used to describe centralized config profiles. These should not be edited.

Parameter	Description
--custom_config_version	Git commit id for Institutional configs. Type: string, Default: master
--custom_config_base	Base directory for Institutional configs. Type: string, Default: https://raw.githubusercontent.com/nf-core/configs/master
--config_profile_name	Institutional config name. Type: string
--config_profile_description	Institutional config description. Type: string
--config_profile_contact	Institutional config contact information. Type: string
--config_profile_url	Institutional config URL link. Type: string

Nextflow Profile Parameters

Parameters to fine-tune your Nextflow setup.

Parameter	Description
--condadir	Directory to Nextflow should use for Conda environments Type: string
--registry	Docker registry to pull containers from. Type: string, Default: dockerhub
--datasets_cache	Directory where downloaded datasets should be stored. Type: string, Default: <BACTOPIA_DIR>/data/datasets
--singularity_cache_dir	Directory where remote Singularity images are stored. Type: string
--singularity_pull_docker_container	Instead of directly downloading Singularity images for use with Singularity, force the workflow to pull and convert Docker containers instead. Type: boolean
--force_rebuild	Force overwrite of existing pre-built environments. Type: boolean
--queue	Comma-separated name of the queue(s) to be used by a job scheduler (e.g. AWS Batch or SLURM) Type: string, Default: general,high-memory
--cluster_opts	Additional options to pass to the executor. (e.g. SLURM: '--account=my_acct_name' Type: string
--container_opts	Additional options to pass to Apptainer, Docker, or Singularityu. (e.g. Singularity: '-D pwd' Type: string
--disable_scratch	All intermediate files created on worker nodes of will be transferred to the head node. Type: boolean

Helpful Parameters

Uncommonly used parameters that might be useful.

Parameter	Description
--monochrome_logs	Do not use coloured log outputs. Type: boolean
--nfdir	Print directory Nextflow has pulled Bactopia to Type: boolean
--sleep_time	The amount of time (seconds) Nextflow will wait after setting up datasets before execution. Type: integer, Default: 5
--validate_params	Boolean whether to validate parameters against the schema at runtime Type: boolean, Default: True
--help	Display help text. Type: boolean
--wf	Specify which workflow or Bactopia Tool to execute Type: string, Default: bactopia
--list_wfs	List the available workflows and Bactopia Tools to use with '--wf' Type: boolean
--show_hidden_params	Show all params when using --help Type: boolean
--help_all	An alias for --help --show_hidden_params Type: boolean
--version	Display version text. Type: boolean

Citations

If you use Bactopia and gtdb in your analysis, please cite the following.

• Bactopia
  Petit III RA, Read TD Bactopia - a flexible pipeline for complete analysis of bacterial genomes. mSystems 5 (2020)

• Genome Taxonomy Database
  Parks DH, Chuvochina M, Rinke C, Mussig AJ, Chaumeil P-A, Hugenholtz P GTDB: an ongoing census of bacterial and archaeal diversity through a phylogenetically consistent, rank normalized and complete genome-based taxonomy Nucleic Acids Research gkab776 (2021)

• GTDB-Tk
  Chaumeil PA, Mussig AJ, Hugenholtz P, Parks DH GTDB-Tk: a toolkit to classify genomes with the Genome Taxonomy Database. Bioinformatics (2019)